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Professor Brian Dixon- A novel application of polyclonal antibodies for det...
Fri. 25 August 2017, 2:00 pm – 3:00 pm AEST
Speaker: Professor Brian Dixon
Affiliation:University of Waterloo
One of the central problems in coral reef ecology is the degree of connectivity between populations; the proportion of larvae that migrate between populations. The high number of offspring released into the water column and the high rates of mortality makes it practically impossible to recapture labeled individuals. Therefore a new application of ELISA (Enzyme-Linked Immunosorbent Assay) is presented here as a feasible and reliable technique to detect coral larvae from plankton samples. Zooplankton samples were collected over nine days at La Parguera, Puerto Rico and Key Largo, Florida. Immunoassays were performed for each of the zooplankton samples and larvae were detected during the spawn but not before or after. The threshold for detection was 3 to 5 larvae per plankton tow (~75 m3). Therefore this ELISA assay will be useful for tracking the movements of planktonic coral larvae and for assessing connectivity between reefs.
About the author:
Professor Brian Dixon was born in Whitehaven Castle in northern England. He received his Bachelor’s degree from Wilfrid Laurier University and his MSc from the University of Guelph. His PhD thesis examined immune responses to parasites of fish and seals and was carried out at Dalhousie University in Halifax, Nova Scotia. Dr. Dixon received a scholarship from the National Science and Engineering Research Council of Canada to be a postdoctoral fellow at Wageningen University in the Netherlands and another from the Medical Research Council of Canada to be a postdoctoral fellow at Stanford University in California. Currently a professor of Biology at the University of Waterloo, Dr. Dixon was the recipient of a Premier’s Research Excellence Award from the Province of Ontario, the Canada Research Chair in Fish and Environmental Immunology and was recently given an Natural Sciences and Engineering Research Council of Canada Synergy award for collaboration with the aquaculture industry.
AIMS Sponsor: Britta Schaffelke