Introduction to: Fluorescence Microscopy, Flow Cytometry, Histology

Actions and Detail Panel

Sales Ended

Sales Have Ended

Registrations are closed
Registration is now closed. Our recording will be available via our website after the event. https://microscopy.unimelb.edu.au/bomp/capabilities/workshops-resources

Event Information

Location

Location

Online event

Sales Have Ended

Registrations are closed
Registration is now closed. Our recording will be available via our website after the event. https://microscopy.unimelb.edu.au/bomp/capabilities/workshops-resources
Event description
This lecture series will provide researchers with an introduction to Histology, Fluorescence Microscopy and Flow Cytometry Applications

About this event

It is aimed at researchers who are new to Histology, Fluorescence Microscopy and Flow Cytometry Applications, or those who would like to refresh their understanding. This event is free to attend and open to all.

Given the possible synergies, this Intro to lecture will be run by

  • Biological Optical Microscopy Platform (BOMP)
  • Melbourne Cytometry Platform (MCP)
  • Melbourne Histology Platform (MHP)

at the University of Melbourne.

This is an online event provided via Zoom. Zoom link will be provided once registered.

A full breakdown of the lectures is shown below:


		Introduction to: Fluorescence Microscopy, Flow Cytometry, Histology image

Lecture 1: Intro to Fluorescence and Fluorescence Microscopy (BOMP)

10am-12pm Monday 3rd May

  • Fluorescence
  • Fluorescence tags, dyes & proteins
  • Fluorescence Microscope

Lecture 2: Introduction to Flow Cytometry and examples of applications (MCP)

10am-12pm Tuesday 4th May

  • Cytometry Platform: Who, what & where?
  • Cytometer architecture
  • Instrument subsystems: Fluidics, Optics & Electronics
  • Nature of the signals (Light Scatter, Fluorescence)
  • Pulse processing
  • Data Visualisation
  • Examples of applications in Cytometry

Lecture 3: Experimental design, acquisition and analysis on Fluorescence Microscopy (BOMP)

10am-12pm Wednesday 5th May

  • Fluorescence Microscopy Applications
  • Experimental design
  • Digital imaging and Image analysis

Lecture 4: Experimental design, acquisition & analysis on Flow Cytometry (MCP)

10am-12pm Friday 7th May

  • Experimental parameters in Cytometry
  • Before you start
  • Sample preparation
  • Instrument setup
  • Compensation and spectral unmixing
  • Panel Design
  • Experimental Controls
  • Data acquisition and analysis
  • Examples of artefacts
  • Cell Sorting

Lecture 5: Introduction to Histology (MHP)

10am-12pm Monday 10th May

  • The aims of histology
  • Overview of the key steps of histology
  • Broad options for sample fixation, embedding, sectioning, and staining
  • Basic introduction to immunostaining

Lecture 6: Experimental design for and applications in histology (MHP)

10am-12pm Tuesday 11th May

  • Integrating histology into experimental design
  • Technology in sample preparation and staining
  • Examples of applications of histology in biomedical and biological research

Location

Online event

Organiser Biological Optical Microscopy Platform

Organiser of Introduction to: Fluorescence Microscopy, Flow Cytometry, Histology

Save This Event

Event Saved